The Hotone Ampero One stands out among multi-effects units because it doesn't have a fixed signal chain. Instead, it allows for easy modification of the effect chain with simple touches and slides on the screen, offering more convenience than traditional "fixed chain" multi-effects or rearranging pedals on a large pedalboard.

Although the Ampero One lacks an input mode selector compared to the original Ampero, it features a specially designed input jack with a high input impedance of up to 4.7M Ohms, ensuring optimal compatibility with a wide range of instruments, including electric guitars, basses, and acoustic instruments, without the need for different input modes.